Service / Technology Instance

About Electron Microscopy, Grenoble, France

The EM platform at IBS provides access to a full range of instruments (three in total) for national and European users via FRISBI and Instruct respectively. This includes classical quality control negative staining experiment (Tecnai 12 EM) prior to setting up cryo conditions (F20 EM equipped for cryo and tomography). Data acquisition can also be carried out on the F20 EM. Our third EM: the Glacios is equipped with a direct electron detector ((Falcon II). The Glacios is able to do single particle imaging and single axis tomo. 

The PF is also offering cellular EM service

Service Availability:

Remote

Physical

Instruments Available:

We offer plastic embbeding ; tokuyasu method; high pressure freezing, immuno-labelling; ultra thin sectionning at room temperature and cryo. The slices can then be observed using a T12 or F20 electron microscope.

Negative staining, Cellular EM, Cryo-EM, cryo-Tomo

Negative staining, cellular EM Orius camera

High resolution single particle and tomography EM. K2 summit direct electron detector. Latitude S software for automated data collection

User Guide

Contact Guy Schoehn: guy.schoehn@ibs.fr

Instruct Centre

Instruct Centre FR2

IBS-ISBG

71 avenue des Martyrs

Grenoble

France

www.ibs.fr & www.isbg.fr

Electron Microscopy, Grenoble, France

Contacts:

Guy Schoehn
Guy Schoehn
CNRS en Alpes
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Eleftherios Zarkadas
Eleftherios Zarkadas
Integrated Structural Biology Grenoble (ISBG)
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